NSJ Bioreagents

EHHADH Antibody / L-bifunctional protein / LBP

Product Code:
 
NSJ-F54986
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the EHHADH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of 1) mouse kidney, 2) rat kidney and 3) rat liver tissue lysate with EHHADH antibody. Predicted molecular weight ~79 kDa.
2 / 4
Western blot testing of mouse 1) liver and 2) kidney tissue lysate with EHHADH antibody. Predicted molecular weight ~79 kDa.
3 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with EHHADH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with EHHADH antibody; Blue=isotype control, Green= EHHADH antibody.

Western blot testing of 1) mouse kidney, 2) rat kidney and 3) rat liver tissue lysate with EHHADH antibody. Predicted molecular weight ~79 kDa.
Western blot testing of mouse 1) liver and 2) kidney tissue lysate with EHHADH antibody. Predicted molecular weight ~79 kDa.
IHC testing of FFPE human hepatocellular carcinoma tissue with EHHADH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with EHHADH antibody; Blue=isotype control, Green= EHHADH antibody.

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CodeSizePrice
NSJ-F54986-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54986-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50
Description:
EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 662-690 from the human protein was used as the immunogen for the EHHADH protein antibody.
Limitation:
This EHHADH antibody is available for research use only.
Localization:
Cytoplasmic (peroxisome)
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q08426