ProSci

GITR Antibody [DTA-1], Rabbit IgG

Product Code:
 
PSI-24-621
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DTA-1
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Agonism
  • Flow Cytometry
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody DTA-1 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of DTA-1 (24-621) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-621- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody DTA-1 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of DTA-1 (24-621) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-621- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

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CodeSizePrice
PSI-24-621-0.2mg0.2mg£681.00
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This product comes from: United States.
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Further Information

Additional Names:
CD357, AITR, GITR-D, Glucocorticoid-induced TNFR-related protein, TNFRSF18, Tumor necrosis factor receptor superfamily member 18
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-mediated suppression of anti-CD3-stimulated T cell proliferation9, and then cloned. This DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a) that showed high neutralizing activity.
NCBI Gene ID #:
21936
NCBI Official Name:
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol:
Tnfrsf18
NCBI Organism:
Mus musculus
Research Area:
Immunology, Cancer
Swissprot #:
O35714
User NOte:
For research use only.