ProSci

Alpha-Tubulin Antibody [F2C], Rabbit IgG

Product Code:
 
PSI-24-365
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
F2C
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Immunohistochemical staining of human cerebral cortex tissue using anti-Alpha Tubulin antibody (F2C </b> Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rmeric version of F2C (24-365). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-365 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration- 2-4 ug/ml.
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<b> Flow-cytometry using the anti-Alpha Tubulin F2C (</b> HepG2 (A)- HeLa (B) and Hek293 (C) cells were stained with unimmunized r antibody (black line) or the rmeric version of F2C (24-365- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
4 / 4
<b> Immunofluoresence staining of fixed HeLa cells with anti-Alpha-tubulin antibody F2C (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of F2C (24-365) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-365- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunohistochemical staining of human cerebral cortex tissue using anti-Alpha Tubulin antibody (F2C </b> Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rmeric version of F2C (24-365). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-365 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration- 2-4 ug/ml.
<b> Flow-cytometry using the anti-Alpha Tubulin F2C (</b> HepG2 (A)- HeLa (B) and Hek293 (C) cells were stained with unimmunized r antibody (black line) or the rmeric version of F2C (24-365- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
<b> Immunofluoresence staining of fixed HeLa cells with anti-Alpha-tubulin antibody F2C (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of F2C (24-365) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-365- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.

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PSI-24-365-0.2mg0.2mg£681.00
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Further Information

Additional Names:
TUBA
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Human MBP (microtubule-binding protein).
NCBI Gene ID #:
7846
NCBI Official Name:
tubulin alpha 1a
NCBI Official Symbol:
TUBA1A
NCBI Organism:
Homo sapiens
Research Area:
Cell Cycle, Cancer, Other
Swissprot #:
Q71U36
User NOte:
For research use only.