ELK Biotechnology

Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit

Product Code:
 
ELK9481
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9481-48T48T£298.00
Quantity:
ELK9481-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
FTO; KIAA1752; fat mass and obesity associated
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Enzyme & Kinase
Sample type:
serum, plasma and other biological fluids
Sensitivity:
0.059 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q9C0B1