ProSci

PH20 Hyaluronidase Recombinant Protein, Tag Free

Product Code:
 
PSI-97-099
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Host Type:
 
Human
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Shipping:
 
Room Temp
Storage:
 
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
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Human PH20, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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The purity of Human PH20, Tag Free is more than 95% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.
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Human PH20, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human PH20, Tag Free is more than 95% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.

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PSI-97-099-0.025mg0.025mg£782.00
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Further Information

Additional Names:
SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N ? acetyl ? beta ? D ? glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Background References:
  • Lin Y., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075.
  • Gmachl M., et al., 1993, FEBS Lett. 336:545-548.
  • Arming S., et al., 1997, Eur. J. Biochem. 247:810-814.
Biological Activity:
The activity of PH20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 ˚C in a 2.0 mL reaction mixture) (QC tested).
Buffer:
Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.
Fusion Tag:
No Tag
NCBI Gene ID #:
6677
NCBI Official Name:
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
NCBI Official Symbol:
SPAM1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
52.6 kDa
Protein Accession #:
AAH26163
Purity:
>95% as determined by SDS-PAGE and >95% as determined by SEC-MALS.
Recombinant Protein Sequence:
Leu 36 - Ser 490
Source:
HEK293 cells
Swissprot #:
P38567