KRIBIOLISA™ Anti-Tremilimumab ELISA

10 ng/ml - 640 ng/ml

Colorimetric, 450nm

Enzyme Immunoassay for quantitative detection of anti-Tremilimumab in serum and plasma. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs enzyme linked immunoassay technique. Standard or samples are pipetted into microwells pre-coated with the drug and anti-drug antibodies present in the sample and standards are bound by the drug. In the second step, HRP conjugate is pipetted and incubated. Free HRP conjugate will be removed by washing. Addition of TMB substrate will develop blue color and and reaction is stopped by addition of stop solution. Colour of the solution turns yellow. Intensity of yellow colour in wells is directly proportional to the concentration of anti-drug antibodies present in Standard or sample. Absorbance is measured at 450 nm.

Serum and Plasma

8.75 ng/ml