Leinco Technologies

Anti-Ly49C (Clone 4LO3311) - PE

Product Code:
 
LEI-L312
Product Group:
 
Primary Antibodies
Host Type:
 
Mouse
Antibody Isotype:
 
IgG3 κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
4LO3311
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Flow Cytometry
Shipping:
 
2-8°C
Storage:
 
This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.
 

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CodeSizePrice
LEI-L312-100ug100 ug£231.00
Quantity:
LEI-L312-200ug200 ug£307.00
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Further Information

Antigen Distribution:
Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells.
Concentration:
0.2 mg/ml
Conjugate/Tag/Label:
R-phycoerythrin (PE)
Format:
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Formulation:
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Long Description:
Ly49 receptors are homodimeric type II C-type lectin-like membrane glycoproteins encoded by a family of highly polymorphic genes in the mouse natural killer (NK) gene complex1. Ly49 receptors recognize class I major histocompatibility complex-I (MHC-1) and MHC-1 like proteins. Ly49C is an inhibitory member of the Ly-49 superfamily. Inhibitory Ly49 receptors are involved in NK cell education1 and play a role in controlling viral infection2. Additionally, Ly49C decreases the activation threshold of NK cells by inhibiting H2-Kb 3. Ly49C is expressed on NK, uterine NK, NKT, and CD8+ Tregs cells1. Ly49C is closely related to Ly49F, Ly49I, and Ly49H3. 4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells4. Hybridoma lines were prepared by fusing immune spleen cells with non-secreting P3X63-Ag8.653 myeloma cells and subsequently screened for production of anti-NK antibodies. Isotype was determined by double-immunodiffusion analysis of hybridoma supernatants using rabbit monospecific anti-mouse immunoglobulins. 4LO3311 is of IgG3, κ isotype. 4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain5. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.
Target:
Ly-49C

References

1. Rahim MM, Tu MM, Mahmoud AB, et al. Front Immunol. 5:145. 2014. 2. Parikh BA, Bern MD, Piersma SJ, et al. Cell Rep. 32(4):107969. 2020. 3. Schenkel AR, Kingry LC, Slayden RA. Front Immunol. 4:90. 2013. 4. Lemieux S, Ouellet-Talbot F, Lusignan Y, et al. Cell Immunol. 134(1):191-204. 1991. 5. Brennan J, Lemieux S, Freeman JD, et al. J Exp Med. 184(6):2085-2090. 1996. 6. Hsu J, Hodgins JJ, Marathe M, et al. J Clin Invest. 128(10):4654-4668. 2018. 7. Depatie C, Chalifour A, Par? C, et al. Int Immunol. 11(9):1541-1551. 1999.