NSJ Bioreagents

PU.1 Antibody / SPI1

Product Code:
 
NSJ-V8999SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PU1/2118
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the PU.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC staining of FFPE human colon carcinoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human Hodgkins Lymphoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human Lymph Node tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE human spleen tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2118) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human colon carcinoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human Hodgkins Lymphoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human Lymph Node tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2118) as confirmation of integrity and purity.

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NSJ-V8999SAF-100UG100ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the PU.1 antibody should be determined by the researcher.
Description:
PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 16-170 was used as the immunogen for the PU.1 antibody.
Limitation:
This PU.1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity
Uniprot #:
P17947

Documents