Recombinant Human IL-18

interleukin 18 (interferon-γ-inducing factor)

Interleukin 18 (IL-18) is an 18-kDa cytokine which identified as a costimulatory factor for production of interferon-g (IFN-g) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24-kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-g is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-g activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-g also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-g. Pleiotropic effects of IL-18 have also been reported, such as, enhancement production of IFN-g and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-g in T cells, enhancement of Fas ligand expression by Th1 cells.

cDNA encoding the matured human IL-18 protein sequence (corresponding to 37-193 aa) was expressed in E. coli.

Less than 0.1 ng/mg of recombinant human IL-18 protein, measured by the LAL assay.

25 ug in PBS containing 0.1% BSAand 1% sucrose. Reconstitute in 250 uL of ice-colddistilled water on ice.

Human: 3606 Mouse: 16173

18 kDa

Greater than 90% purity as confirmed on SDS-PAGE by Coomassie brilliant blue staining.

2 years

IL-18