Anti-HP1alpha (Human) pAb

WB - 0.25-1 ug/mL (chemiluminescence detection system) IP - 1 ug/200 uL of cell extract from 5x106 cells ICC - 10 ug/mL IHC - 10 ug/mL (Heat treatment required for paraffin)

HP-1α is a nuclear protein present in centromeric and pericentromeric heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic gene silencing. HP-1α is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. HP-1α also interacts with lamin B receptor, contributing to the association of the heterochromatin with the inner nuclear membrane. Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure that occur at various times during the cell cycle.

50 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Human: 23468 Mouse: 12419

Synthetic peptide (175-191 aa of human HP1alpha) (CHAYPEDAENKEKETAKS)

This antibody reacts with human and mouse HP1 a (29 kDa) on Western blotting.

1 year

This antibody was an affinity chromatography purified rabbit polyclonal antibody raised against synthesized peptide, CHAYPEDAENKEKETAKS, which corresponding to human HP1 a (175-191 a.a.).

HP1a