MBL

Anti-BAX (Human) mAb

Product Code:
 
MBL-M010-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
4F11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
4°C
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MBL-M010-3100 ug£296.00
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  • Further Information
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Further Information

Applications:
WB - 0.1-1 ug/mL (chemiluminescence detection system) IP - 5-10 ug /200 uL of cell extract from 5x106 cells FCM - 5-10 ug/mL (final concentration) ICC - 10 ug/mL IHC - 10 ug/mL (Heat treatment required for paraffin)
Background:
Bax (Bcl-associated X protein) is a 21 kDa tumor suppressor protein that suppresses tumorigenesis and stimulates apoptosis in vivo. Bax has extensive amino acid homology to Bcl-2. It can homodimerize through its BH3 domain and it forms heterodimers with other Bcl-2 family members through its BH1 and BH2 domains. Overexpression of Bax promotes apoptosis and counters the death repressor activity of Bcl-2 and Bcl-xL. It is believed that the ratio of Bcl-2/Bax complexes to free protein controls the relative susceptibility of cells to death stimuli. Apoptotic stimuli cause the translocation of monomeric Bax from the cytosol to the mitochondria where it forms Bax homodimers. Localization of Bax to the mitochondria results in the activation of caspase-3, membrane blebbing, and nuclear fragmentation. Bax also induces mitochondrial dysfunction by increasing mitochondrial membrane permeability. It accelerates the opening of the mitochondrial porin channel VDAC, thus regulating the release of cytochrome c during apoptosis.
Formulation:
This antibody is lyophilized form.Prepare a stock solution by dissolving the lyophilizedantibody in 100 ul of distilled water. After reconstitution,the IgG concentration should be 1 mg/mL in PBS (pH 7.2)containing 1% sucrose. No preservative is contai
Gene IDs:
Human: 581 Mouse: 12028
Immunogen Translated:
Recombinant human BAX protein
Reactivity:
This antibody reacts with human BAX (24 kDa) on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 4F11) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with recombinant human BAX.
Target:
Bax

Documents

References

1) Cartron, P-F., et al., J. Biol. Chem. 280, 10587-10598 (2005) 2) Starczynski, J., et al., J. Clin. Oncol. 23, 1514-1521 (2005) 3) Su?rez, L., et al., Clin. Cancer Res. 10, 7599-7606 (2004) 4) Utsunomiya, K., et al., J. Histochem. Cytochem. 52, 805-812 (2004) 5) Cartron, P-F., et al., J. Biol. Chem. 278, 11633-11641 (2003) 6) Sturlan, S., et al., Blood 101, 4990-4997 (2003) 7) Than, N. G., et al., Eur. J. Biochem. 270, 1176-1188 (2003) 8) Lataillade, J-J., et al., Blood 99, 1117-1129 (2002) 9) Nomura, M., et al., Cancer Res. 59, 5542-5548 (1999) 10) De Saint Jean, M., et al., Invest. Ophthalmol. Vis. Sci. 40, 2199-2212 (1999) 11) Penault-Llorca, F., et al., Pathol. Res. Pract. 194, 457-464 (1998) Clone 4F11 is used in these references.