MBL

Anti-FADD (Human) mAb

Product Code:
 
MBL-M033-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1F7
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Application:
 
Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 2
2 / 2
❮❯

No additional charges, what you see is what you pay! *

CodeSizePrice
MBL-M033-3100 ug£296.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • References
  • Show All

Further Information

Applications:
WB - 1 ug/mL (chemiluminescence detection system)
Background:
FADD (Fas-associated death domain protein)/MORT1 has been identified as a protein that associates specifically with the intracellular domain of Fas. It contains death domain (DD) and death effecter domain (DED) as well. DD is found in several death-inducing receptors of the TNF family, including Fas (CD95/APO-1) and TNFR-1. DED is found at N-terminus of FADD and it also present within the ICE-like protease, caspase-8 (FLICE/Mch5/MACH) and caspase-10 (FLICE2). Both DD and DED are able to associate with homologous regions in other proteins, and thus prompt binding of such proteins to one another. Upon activation Fas, it relays death signals through DD, which interacts with the DD of the adaptor molecules FADD, recruiting them to the membrane. FADD then associates with caspase-8 through DED, leading to the assembly of a death-inducing signaling complex (DISC). DISC-associated caspase-8 subsequently initiates proteolytic activation of other caspase, which in turn leads to apoptosis.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 8772 Mouse: 14082
Immunogen Translated:
Length Recombinant human FADD (1-208 a.a.)
Reactivity:
This antibody reacts with human and mouse FADD on Western blotting. Clone 4G3 (MBLI; code no. M035-3) and 1F7 recognize different epitope sites.
Shelf Life:
1 year
Source:
This antibody was purified from hybridoma (clone 1F7) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant full-length human FADD (1-20°8 aa).
Target:
FADD

Documents

References

1) Tsuruma, K., et al., J. Biol. Chem. 281, 11397-11404 (2006) 2) Miyaji, M., et al., J. Exp. Med. 202, 249-259 (2005) 3) Cui, H., et al., J. Biol. Chem. 280, 9474-9481 (2005) 4) Robbins, M. A., et al., Endocrinology 144, 4616-4625 (2003) 5) Kabsch, K., et al., J. Virol. 76, 12162-12172 (2002) 6) Medema, J., et al., EMBO J. 16, 2794 (1997) 7) Muzio, M., et al., Cell 85, 817-827 (1996) 8) Boldin, M. P., et al., Cell 85, 803-815 (1996) Clone 1F7 is used in the reference 1) - 5).