MBL

Anti-Hsp40 (Human) mAb

Product Code:
 
MBL-M039-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2E1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
4°C
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MBL-M039-3100 ug£323.00
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  • Further Information
  • Documents
  • References
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Further Information

Applications:
WB - 1-2 ug/mL (chemiluminescence detection system) IP - 2-5 ug/200 uL of cell extract from 5 x 106 cells
Background:
40 kDa heat shock protein (hsp40) is a novel heat shock protein induced by heat shock and other stresses in mammalian and avian cells. The cDNA sequence of the hsp40 is identical with HDJ1. Deduced amino acid sequence of the hsp40 cDNA is homologues to DnaJ, an E. coli heat shock protein and its homologues in yeast such as SCJ1, YDJ1 (MAS5), SIS1, SEC63 and zuotin. E coli?s DnaJ heat shock protein is known to function together with DnaK (hsp70) and GrpE as a molecular chaperone, which is necessary for assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. The hsp40 is colocarized with hsp70 in the nuclei and nucleoli of heat-shocked HeLa cells which suggests that hsp70 (DnaK)-hsp40 (DnaJ) chaperone system is ubiquitous.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained.
Gene IDs:
Human: 3337 Mouse: 81489
Immunogen Translated:
Human full-length Hsp40 (1-340 a.a.)
Reactivity:
This antibody recognizes J domain (1-70° aa) of hsp40 and reacts with hsp40 on Western blotting and Immunoprecipitation (40 kDa).
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma (clone 2E1) was established by fusion of mouse myeloma cell P3U1 with Balb/c splenocyte immunized with full-length human HSP40 (1-340 aa).
Target:
HSP40

Documents

References

1) Helmlinger, D., et al., Hum. Mol. Genet. 11, 3351-3359 (2002) 2) Sugito, K., et al., FEBS Letter 358, 161-164 (1995) 3) Frydman, J., et al., Nature 370, 111-117 (1994) 4) Ohtsuka, K. et al., Exp. Cell Res. 209, 357-366 (1993) 5) Hattri, H., et al., J. of Cell Sci. 104, 629-638 (1993) 6) Ohtsuka, K., Biochem. Biophys. Res. Commun. 197, 235-240 (1993) 7) Hattori, H., et al., Cell Struct. Funct. 17, 77-86 (1992) 8) Ohtsuka, K., et al., Biochem. Biophys. Res. Commun. 166, 642-647 (1990) Clone 2E1 is used in reference number 1).