MBL

Anti-DNA Topoisomerase IIα (Human) mAb

Product Code:
 
MBL-M042-3
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1C5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
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MBL-M042-3100 ug£323.00
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  • Further Information
  • Documents
  • References
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Further Information

Applications:
WB - 0.1-0.5 ug/mL (chemiluminescence detection system) IP - 1-10 ug/200-300 uL of cell extract FCM - 0.1-1 ug/mL (final concentration) ICC - 1-10 ug/mL IHC - 1 ug/mL (Heat treatment required for paraffin)
Background:
Topoisomerase II (Topo II) is a nuclear enzyme that regulates the topological states of DNA by transient breakage and rejoining double-stranded DNA, catalyzing the decatenation and unknotting of topologically linked DNA circles and the relaxation of supercoiled DNA. In mammalian cells, Topo II consists of two isozymes, Topo IIα (170 kDa) and Topo IIα (180 kDa). Expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo IIα is expressed constantly throughout cell cycle, whereas the expression of Topo IIα is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. It is considered that Topo IIα plays an essential role in cell proliferation, especially during late S to M phase.
Concentration:
1 mg/mL
Formulation:
100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative.
Gene IDs:
Human: 7153 Mouse: 21973
Immunogen Translated:
Recombinant human DNA Topo IIα (182a.a. terminal C)
Reactivity:
This antibody detects 170 kDa of human Topo IIα on Western blotting with total cell lysate from human cell lines.
Shelf Life:
1 year
Source:
This antibody was purified from mouse ascites fluid using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the recombinant human Topoisomerase IIα protein corresponding to C-terminal 182 aa.

Documents

References

1) Nakagawa, T., et al., Cancer Res. 64, 4826-4832 (2004) 2) Mensah-Osman, E.J., et al., Mol. Cell Ther. 14, 1321-1326 (2002) 3) Escargueil, A.E., et al., J. Biol. Chem. 275, 34710-34718 (2000) 4) Meyer, K. N., et al., J. Cell Biol. 136, 775-788 (1997) 5) Sasano, K., et al., Cell Technology (in japanese) 16, 1613-1617 (1997) 6) Goswami, P.C., et al., Mol. Cell. Biol. 16, 1500-1508 (1996) 7) Woessner, R.D., et al., Cell Growth Differ. 2, 209-214 (1991) Clone 1C5 is used in the reference number 1).