Elabscience

Recombinant p53 Monoclonal Antibody

Product Code:
 
E-AB-81592
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
R07-6A7
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot detection of p53 in Jurkat cell lysates using p53 Rabbit mAb(1:500 diluted).Predicted band size:44kDa.Observed band size:53kDa.
2 / 2
Immunofluorescence of p53 (green) in MCF-7 using p53 antibody at dilution 1:20, and DAPI(blue)
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Western blot detection of p53 in Jurkat cell lysates using p53 Rabbit mAb(1:500 diluted).Predicted band size:44kDa.Observed band size:53kDa.
Immunofluorescence of p53 (green) in MCF-7 using p53 antibody at dilution 1:20, and DAPI(blue)

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CodeSizePrice
E-AB-81592-50uL50uL£184.00
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E-AB-81592-100uL100uL£251.00
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Further Information

Abbreviation:
p53
Background:
TP53,also named as P53 and NY-CO-13,belongs to the p53 family. It has 9 isoforms. In SDS-Page,the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.
Buffer:
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Calculated MW:
44kDa
Concentration:
300 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000 IF 1:20-1:100
Immunogen:
Recombinant protein of human p53
ObservedMW:
53kDa
Purification method:
Affinity Purified
Target Synonym:
Antigen NY-CO-13;BCC7;Cellular tumor antigen p53;FLJ92943;LFS1;Mutant tumor protein 53;p53;p53 tumor suppressor;P53;Phosphoprotein p53;Tp53;Transformation related protein 53;TRP53;Tumor protein 53;Tumor protein p53;Tumor suppressor p53
UNIProt ID:
P04637

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0223523422001350