Elabscience

Recombinant E.coli Tryptophan Synthase Protein (His Tag)

Product Code:
 
PKSQ050057
Product Group:
 
Recombinant Proteins
Supplier:
 
Elabscience
Host Type:
 
E.coli
Regulatory Status:
 
RUO
Shipping:
 
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt store it immediately at < -20°C.
Storage:
 
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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CodeSizePrice
PKSQ050057-10ug10ug£297.00
Quantity:
PKSQ050057-50ug50ug£498.00
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Further Information

Abbreviation:
Tryptophan Synthase
Accession:
P0A877&P0A879
Background:
Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3-phosphate (IGP) to give indole and D-glyceraldehyde 3-phosphate (G3P). Indole is then transferred through a 25- hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.
Calculated MW:
72.5 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Supplied as a 0.2 um filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
N-His
ObservedMW:
28&40-50 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met1-Ser268&Thr2-Ile397
Target Synonym:
Tryptophan synthetase;Tryptophan synthase
UNIProt ID:
P0A877&P0A879

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