Elabscience

RPS27A Polyclonal Antibody

Product Code:
 
E-AB-60562
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunohistochemistry of paraffin-embedded Rat kidney using RPS27A Polyclonal Antibody at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using RPS27A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded Rat kidney using RPS27A Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RPS27A Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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E-AB-60562-60uL60uL£210.00
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E-AB-60562-120uL120uL£291.00
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E-AB-60562-200uL200uL£431.00
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Further Information

Abbreviation:
RPS27A
Background:
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
17kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human RPS27A (NP_001170884.1).
ObservedMW:
14kDa
Purification method:
Affinity purification
Target Synonym:
RPS27A;CEP80;HEL112;S27A;UBA80;UBC;UBCEP1;UBCEP80
UNIProt ID:
P62979

Documents