Elabscience

RBFOX3 Polyclonal Antibody

Product Code:
 
E-AB-70267
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunohistochemistry analysis of paraffin-embedded mouse brain using RBFOX3 Polyclonal Antibody at dilution of 1:500.
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Immunohistochemistry analysis of paraffin-embedded mouse brain using RBFOX3 Polyclonal Antibody at dilution of 1:500.

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  • Further Information
  • Documents
  • References
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Further Information

Abbreviation:
RBFOX3
Background:
Immunoprecipitation and mass spectrometry of the two major NeuN species at 45?50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism.
Buffer:
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Calculated MW:
46-48kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000, IHC 1:300-1:800
Immunogen:
KLH conjugated Synthetic peptide corresponding to NeuN
ObservedMW:
46-48kDa
Purification method:
Affinity purification
Target Synonym:
FLJ56884;FLJ58356;Fox-1 homolog C;fox1 homolog C;Fox3;FOX3NeuN;hexaribonucleotide binding protein 3;HRNBP3;NEUN;neuronal nuclei;Rbfox3;RFOX3;RNA binding protein fox-1 homolog 3;RNA binding protein;fox 1 homolog (C. elegans) 3
UNIProt ID:
Q8BIF2

Documents

References

  • https://academic.oup.com/jnen/article-abstract/81/8/596/6633607