Elabscience

PDHA1 Polyclonal Antibody

Product Code:
 
E-AB-60493
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Immunohistochemistry of paraffin-embedded Human colon carcinoma using PDHA1 Polyclonal Antibody at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using PDHA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded Human colon carcinoma using PDHA1 Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using PDHA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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E-AB-60493-60uL60uL£210.00
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E-AB-60493-120uL120uL£291.00
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E-AB-60493-200uL200uL£431.00
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Further Information

Abbreviation:
PDHA1
Background:
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
40kDa/43kDa/44kDa/47kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human PDHA1 (NP_000275.1).
ObservedMW:
45kDa
Purification method:
Affinity purification
Target Synonym:
PDHA1;PDHA;PDHAD;PDHCE1A;PHE1A
UNIProt ID:
P08559

Documents