Elabscience

Recombinant Human Fibronectin/FN Protein (Active)

Product Code:
 
PKSH032450
Product Group:
 
Recombinant Proteins
Supplier:
 
Elabscience
Host Type:
 
Human
Regulatory Status:
 
RUO
Application:
 
Cell Culture
Shipping:
 
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
 
Lyophilized protein should be stored at < -20°C though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
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CodeSizePrice
PKSH032450-10ug10ug£106.00
Quantity:
PKSH032450-50ug50ug£155.00
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Abbreviation:
Fibronectin;FN
Accession:
P02751
Activity:
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
Background:
Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains;fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment; is a protein of ~63 kDa containing a central cell-binding domain; a high affinity heparin-binding domain II;and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand; a CS-I site; and a VLA-5 ligand; a cell attachment domain; and virus vectors binds to a heparin binding domain II; which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors; and facilitates the gene transduction in the result.
Calculated MW:
62.7 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
E.coli
Formulation:
Lyophilized from a 0.2 um filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 6.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
None
ObservedMW:
60 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Pro1270-Ser1546&Ala1721-Thr2016
Target Synonym:
Fibronectin;FN1;CIG;ED-B;FINC;FN;FNZ;GFND;GFND2;LETS;MSF
UNIProt ID:
P02751

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