Elabscience

Flag-Tag Polyclonal Antibody

Product Code:
 
E-AB-20045
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western Blot analysis of 1ug Flag fusion protein using Flag-Tag Polyclonal Antibody at dilution of 1:1000.
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Western Blot analysis of 1ug Flag fusion protein using Flag-Tag Polyclonal Antibody at dilution of 1:1000.

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CodeSizePrice
E-AB-20045-30uL30uL£125.00
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E-AB-20045-60uL60uL£146.00
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E-AB-20045-120uL120uL£196.00
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E-AB-20045-200uL200uL£216.00
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This product comes from: China.
Typical lead time: 14-21 working days.
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  • Further Information
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Further Information

Abbreviation:
Flag-Tag
Background:
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.
Buffer:
PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:5000-20000
Immunogen:
Synthetic Peptide
Purification method:
Affinity purification
Target Synonym:
DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0896841122001834