Elabscience

Rat TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Product Code:
 
E-EL-R0939
Product Group:
 
ELISA Kits
Supplier:
 
Elabscience
Host Type:
 
Rat
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Ice packs
Storage:
 
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
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Standard curve
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Standard curve

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CodeSizePrice
E-EL-R0939-24T24T£207.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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E-EL-R0939-48T48T£321.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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E-EL-R0939-96T96T£403.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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This product comes from: China.
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  • Further Information
  • Documents
  • References
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Further Information

Abbreviation:
TRACP-5b
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Rat TRACP-5b in samples. No significant cross-reactivity or interference between Rat TRACP-5b and analogues was observed
Detection Range:
0.63-40 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.38 ng/mL
Target Species:
Rat
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Rat TRACP-5b. You can calculate the concentration of Rat TRACP-5b in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P29288
Usage:
This ELISA kit applies to the in vitro quantitative determination of Rat TRACP-5b concentrations in serum, plasma and other biological fluids.

Documents

References

  • https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-00976-4
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579055/
  • https://www.sciencedirect.com/science/article/pii/S0024320522001060
  • https://www.mdpi.com/648596
  • https://www.sciencedirect.com/science/article/pii/S0753332220304960
  • https://www.sciencedirect.com/science/article/pii/S0960076015300157
  • https://link.springer.com/article/10.1007/s12020-022-03199-1
  • https://josr-online.biomedcentral.com/articles/10.1186/s13018-022-03162-w
  • https://journals.sagepub.com/doi/abs/10.1136/acupmed-2016-011268
  • https://portlandpress.com/bioscirep/article-abstract/35/4/e00239/56188
  • https://www.spandidos-publications.com/10.3892/etm.2015.2553
  • https://link.springer.com/article/10.1007/s13596-019-00377-x