Elabscience

Human CFTR(Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit

Product Code:
 
E-EL-H1766
Product Group:
 
ELISA Kits
Supplier:
 
Elabscience
Host Type:
 
Human
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
Ice packs
Storage:
 
An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
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Standard curve

Standard curve

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CodeSizePrice
E-EL-H1766-24T24T£207.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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E-EL-H1766-48T48T£321.00
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E-EL-H1766-96T96T£403.00
Also available in 96T*5 and 96T*10 pack sizes, contact us for bulk pricing!
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This product comes from: China.
Typical lead time: 14-21 working days.
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  • Further Information
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Further Information

Abbreviation:
CFTR
Assay Time:
3.5h
Cross reactivity:
This kit recognizes Human CFTR in samples. No significant cross-reactivity or interference between Human CFTR and analogues was observed
Detection Range:
0.16-10 ng/mL
Detection Type:
Colormetric
Precision:
Both intra-CV and inter-CV are < 10%.
Recovery rate:
80%-120%
Sensitivity:
0.1 ng/mL
Target Species:
Human
Test Principle:
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFTR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CFTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFTR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The OD value is proportional to the concentration of Human CFTR. You can calculate the concentration of Human CFTR in the samples by comparing the OD of the samples to the standard curve.
Tested Sample Types:
serum, plasma and other biological fluids
Type:
Sandwich
UNIProt ID:
P13569
Usage:
This ELISA kit applies to the in vitro quantitative determination of Human CFTR concentrations in serum, plasma and other biological fluids.

Documents

References

  • https://www.nature.com/articles/s41598-018-34960-0
  • https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.28156