Elabscience

Reactive Oxygen Species (ROS) Fluorometric Assay Kit

Product Code:
 
E-BC-K138-F
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
Reagent1:-20°C;Others:2-8°C
 

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E-BC-K138-F-96T96T£224.00
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This product comes from: China.
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  • Further Information
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Further Information

Assay Time:
60 min
Detection Instrument:
Fluorescence Microplate reader, Fluorescence Microscope, Flow Cytometry
Detection Type:
Fluorescence method
Result Type:
Cell-based (quantitative)
Target Synonym:
ROS;DCFH-DA;DCFH;DCF
Test Principle:
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Tested Sample Types:
Fresh tissue,cultured cells

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S2211383522004798
  • https://www.sciencedirect.com/science/article/pii/S0043135420312884
  • https://www.sciencedirect.com/science/article/pii/S0891584922009455
  • https://www.mdpi.com/1983336
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.14085
  • https://journals.asm.org/doi/abs/10.1128/Spectrum.00074-21
  • https://www.sciencedirect.com/science/article/pii/S0147651322009770
  • https://www.ahajournals.org/doi/abs/10.1161/hypertensionaha.118.11035
  • https://www.tandfonline.com/doi/abs/10.1080/10717544.2022.2147280
  • https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/biof.1824
  • https://journals.sagepub.com/doi/abs/10.1177/0271678X211029060
  • https://static.frontiersin.org/articles/10.3389/fmolb.2022.816510/full