Elabscience

Recombinant Human R-Spondin 1/RSPO1 Protein (His Tag)(Active)

Product Code:
 
PKSH031269
Product Group:
 
Recombinant Proteins
Supplier:
 
Elabscience
Host Type:
 
Human
Regulatory Status:
 
RUO
Applications:
  • Cell Culture
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
 
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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PKSH031269-100ug100ug£862.00
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Further Information

Abbreviation:
R-Spondin 1;RSPO1
Accession:
NP_001033722.1
Activity:
1. Immobilized human RSPO1 at 20 ug/ml (100 ul/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml. 2. Immobilized human RSPO1 at 20 ug/ml (100 ul/well) can bind mouse CD36 with a linear ranger of 6. 4-800 ng/ml. 3. Measured by its ability to induce activation of ?catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 ug/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Background:
RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Calculated MW:
28.2 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
42 kDa
Purity:
> 95 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Ala 263
Target Synonym:
RSPO1;R-spondin1;RP11-566C13.1;CRISTIN3;FLJ40906;RSPO Rspo1;R-spondin;Rspondin;RP23-325M14.2;Roof plate-specific spondin-1

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