Elabscience

Recombinant Human CD31/PECAM1 Protein (His Tag)(Active)

Product Code:
 
PKSH031833
Product Group:
 
Recombinant Proteins
Supplier:
 
Elabscience
Host Type:
 
Human
Regulatory Status:
 
RUO
Applications:
  • Cell Culture
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
 
This product is provided as lyophilized powder which is shipped with ice packs.
Storage:
 
Generally lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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PKSH031833-100ug100ug£441.00
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This product comes from: China.
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Further Information

Abbreviation:
CD31;PECAM1
Accession:
EAW94208.1
Activity:
Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8x104 cells/well are added to CD31-His coated plates (5ug/mL, 100 uL/well) in the presence of 10 ug/mL PHA, approximately > 70% will adhere after 30 minutes at 37°C.
Background:
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
Calculated MW:
66 kDa
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method.
Expression Host:
HEK293 Cells
Formulation:
Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Fusion tag:
C-His
ObservedMW:
100-110 kDa
Purity:
> 97 % as determined by reducing SDS-PAGE.
Sequence:
Met 1-Lys 601
Target Synonym:
Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA;PECA1;CD31;PECAM1

Documents