ALK Antibody / Anaplastic Lymphoma Kinase

Western blot: 1-2ug/ml

Optimal dilution of the Anaplastic Lymphoma Kinase antibody should be determined by the researcher.

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A portion of amino acids 220-335 was used as the immunogen for the Anaplastic Lymphoma Kinase antibody.

This Anaplastic Lymphoma Kinase antibody is available for research use only.

Cytoplasmic, Nuclear

Protein A/G affinity

Q9UM73