NSJ Bioreagents

Desmin Antibody

Product Code:
 
NSJ-V9768SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
DES/1711
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant Desmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
2 / 3
IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
3 / 3
SDS-PAGE Analysis of Purified, BSA-Free recombinant Desmin Antibody (clone rDES/1711). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human uterus with recombinant Desmin antibody (clone rDES/1711). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free recombinant Desmin Antibody (clone rDES/1711). Confirmation of Integrity and Purity of the Antibody.

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NSJ-V9768SAF-100UG100ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Desmin antibody should be determined by the researcher.
Description:
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain α-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human Desmin protein was used as the immunogen for the recombinant Desmin antibody.
Limitation:
This recombinant Desmin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P17661

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