NSJ Bioreagents

Granzyme B Antibody

Product Code:
 
NSJ-V2564SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Western blot analysis of human stomach lysate using Granzyme B antibody. Predicted molecular weight: 29-37 kDa.

Western blot analysis of human stomach lysate using Granzyme B antibody. Predicted molecular weight: 29-37 kDa.

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CodeSizePrice
NSJ-V2564SAF-100UG100 ug£534.00
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Application Note:
Optimal dilution of the Granzyme B antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Description:
Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.
Limitation:
This Granzyme B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Uniprot #:
P10144

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