TRP1 / Tyrosinase-Related Protein-1 Antibody

Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the TRP1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Recombinant full-length human protein was used as the immunogen for the TRP1 antibody.

This TRP1 antibody is available for research use only.

Cytoplasmic

Protein G affinity chromatography

P17643