CEA Antibody / Carcinoembryonic Antigen

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Optimal dilution of the anti-CEA antibody to be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human colon carcinoma extract was used as the immunogen for the anti-CEA antibody.

This anti-CEA antibody is available for research use only.

Cytoplasmic and luminal surface

Protein G affinity chromatography

P06731