NSJ Bioreagents

CD31 Antibody / PECAM-1

Product Code:
 
NSJ-V7214SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
RMCD31-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone RMCD31-1). Expected molecular weight: 83-130 kDa depending on level of glycosylation.~
2 / 2
IHC staining of FFPE human angiocarcinoma with recombinant CD31 a
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Western blot testing of human ThP1 cell lysate with recombinant CD31 antibody (clone RMCD31-1). Expected molecular weight: 83-130 kDa depending on level of glycosylation.~
IHC staining of FFPE human angiocarcinoma with recombinant CD31 a

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NSJ-V7214SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the recombinant CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
5175
Immunogen:
A full length recombinant human protein was used as the immunogen for the recombinant CD31 antibody.
Limitation:
This recombinant CD31 antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein A affinity

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