NSJ Bioreagents

Estrogen Receptor beta 5 Antibody

Product Code:
 
NSJ-V8669SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PPG5/25
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Estrogen Receptor beta 5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human breast with Estrogen Receptor beta 5 antibody (clone PPG5/25). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Es
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IHC staining of FFPE human breast with Estrogen Receptor beta 5 antibody (clone PPG5/25). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free Es

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NSJ-V8669SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the Estrogen Receptor beta 5 antibody should be determined by the researcher.
Description:
This MAb specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). No cross-reactivity is seen with estrogen receptor beta 1 or 2 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids LLSHVRHARYAP from the C-terminus of human Estrogen Receptor beta 5 were used as the immunogen for the Estrogen Receptor beta 5 antibody.
Limitation:
This Estrogen Receptor beta 5 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q92731

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