NSJ Bioreagents

MUC1 Antibody / Mucin-1

Product Code:
 
NSJ-V2371IHC
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MUC1/845
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the EMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).
2 / 5
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EMA antibody (MUC1/845).
3 / 5
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
4 / 5
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
5 / 5
SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.

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NSJ-V2371IHC-7ML7 ml£534.00
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This product comes from: United States.
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Further Information

Application Details:
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.

1. HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Format:
Purified
Formulation:
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Gene ID #:
4582
Immunogen:
Human milk fat globule membranes were used as the immunogen for this EMA antibody.
Limitation:
This EMA antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography

Documents