NSJ Bioreagents

MCM7 Antibody

Product Code:
 
NSJ-V3365SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MCM7/1466
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human samples with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.~
2 / 2
Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected mol
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Western blot testing of human samples with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.~
Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected mol

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NSJ-V3365SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
Limitation:
This MCM7 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P33993

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