NSJ Bioreagents

CD137L Antibody / 4-1BBL / TNFSF9

Product Code:
 
NSJ-V8184SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CD137L/1547
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD137L antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC staining of FFPE human renal cell carcinoma with CD137L antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human renal cell carcinoma with CD137L antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 4
Flow cytometry testing of PFA-fixed human 293 cells with CD137L antibody; Red=isotype control, Blue= CD137L antibody.
4 / 4
SDS-PAGE analysis of purified, BSA-free CD137L antibody as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human renal cell carcinoma with CD137L antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma with CD137L antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Flow cytometry testing of PFA-fixed human 293 cells with CD137L antibody; Red=isotype control, Blue= CD137L antibody.
SDS-PAGE analysis of purified, BSA-free CD137L antibody as confirmation of integrity and purity.

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NSJ-V8184SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
TNFSF9 / 4-1BBL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9 / 4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9 / 4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. It has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine is required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant full-length human CD137L protein was used as the immunogen for the CD137L antibody.
Limitation:
This CD137L antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P41273

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