Cyclin A2 Antibody / CCNA2

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.

This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

890

Full length protein was used as the immunogen for this CCNA2 antibody.

This Cyclin A2 antibody is available for research use only.

Nuclear, cytoplasmic

Protein G affinity chromatography

P20248