NSJ Bioreagents

CD86 Antibody

Product Code:
 
NSJ-V3555SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
C86/2160R
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC testing of FFPE human colon with recombinant CD86 antibody (C86/2160R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
IF/ICC staining of human Raji cells with recombinant CD86 antibody (green) and Reddot nuclear stain (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone C86/2160R) as confirmation of integrity and purity.
❮❯

IHC testing of FFPE human colon with recombinant CD86 antibody (C86/2160R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
IF/ICC staining of human Raji cells with recombinant CD86 antibody (green) and Reddot nuclear stain (red).
SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone C86/2160R) as confirmation of integrity and purity.

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NSJ-V3555SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant CD86 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human full-length protein was used as the immunogen for the recombinant CD86 antibody.
Limitation:
This recombinant CD86 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein A affinity chromatography
Uniprot #:
P42081

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