NSJ Bioreagents

EIF4E Antibody

Product Code:
 
NSJ-F54054
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse lgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8C8-C10-C12
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
Store the EIF4E antibody at -20oC.
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Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.~
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Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.~

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NSJ-F54054-0.1ML0.1 ml£502.00
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Further Information

Application Details:
Western blot: 1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt]
Format:
Purified
Formulation:
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Immunogen:
A human recombinant protein was used as the immunogen for this EIF4E antibody.
Limitation:
This EIF4E antibody is available for research use only.
Purity:
Protein G affinity
Uniprot #:
P06730

Documents