NSJ Bioreagents

CD32 Antibody / FCGR2A

Product Code:
 
NSJ-V2496SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8.7
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Functional Study
  • Immunofluorescence (IF)
Storage:
 
Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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SDS-PAGE Analysis of Purified, BSA-Free CD32 Antibody (clone 8.7). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free CD32 Antibody (clone 8.7). Confirmation of Integrity and Purity of the Antibody.

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NSJ-V2496SAF-100UG100 ug£534.00
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Further Information

Application Details:
Functional studies (order BSA/sodium azide-free format),Immunofluorescence: 0.5-1ug/ml
Application Note:
Optimal dilution of the CD32 antibody should be determined by the researcher.
Description:
This mAb reacts with a CD32 (FcgRII) epitope distinct from that defined by mAb 8.26 and the epitope overlaps with that of mAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.
Limitation:
This CD32 antibody is available for research use only.
Localization:
Cytoplasm, plasma membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P12318

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