Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His)

QHD43416.1

2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein

35-43kDa, reducing conditions

The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Immobilized SARS-CoV-2 S Protein RBD-SD1-His (Cat#DRA42) at 5ug/ml (100 ul/well) can bind Human ACE-2-Fc(Cat#C05Y).The ED50 of Recombinant Human ACE-2-Fc(Cat#C05Y) is 76 ng/ml.

31.4kDa

Greater than 95% as determined by reducing SDS-PAGE/SEC-HPLC.