NSJ Bioreagents

FGFR3 Antibody / Fibroblast Growth Factor Receptor 3 (Antigen affinity purified)

Product Code:
 
NSJ-RQ6767
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Fibroblast Growth Factor Receptor 3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human Caco-2 cells with Fibroblast Growth Factor Receptor 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) A549, 2) HeLa, 3) HepG2 and 4) HEK293 cell lysate with Fibroblast Growth Factor Receptor 3 antibody. Predicted molecular weight: 87-135 kDa depending on glycosylation level.
3 / 3
Flow cytometry testing of human 293T cells with Fibroblast Growth Factor Receptor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibroblast Growth Factor Receptor 3 antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with Fibroblast Growth Factor Receptor 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A549, 2) HeLa, 3) HepG2 and 4) HEK293 cell lysate with Fibroblast Growth Factor Receptor 3 antibody. Predicted molecular weight: 87-135 kDa depending on glycosylation level.
Flow cytometry testing of human 293T cells with Fibroblast Growth Factor Receptor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibroblast Growth Factor Receptor 3 antibody.

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CodeSizePrice
NSJ-RQ6767-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Fibroblast Growth Factor Receptor 3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Fibroblast growth factor receptor 3, also known as CD333, is a protein that in humans is encoded by the FGFR3 gene. This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. The FGFR3 gene is mapped to the HD region on chromosome 4p16.3. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids EGPTLANVSELELPADPKWELSRARLTLGK from the human protein were used as the immunogen for the Fibroblast Growth Factor Receptor 3 antibody.
Limitation:
This Fibroblast Growth Factor Receptor 3 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity purified
Uniprot #:
P22607

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