NSJ Bioreagents

KRT7 Antibody / Cytokeratin 7

Product Code:
 
NSJ-V4278
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KRT7/3733
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Cytokeratin 7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human SK-BR-3 cell lysate with Cytokeratin-7 antibody (clone KRT7/3733).~
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IHC staining of FFPE human bladder carcinoma with Cytokeratin-7 antibody (clone KRT7/3733). HIER: boil tissue sections in pH 9 10mM Tris with
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Western blot testing of human SK-BR-3 cell lysate with Cytokeratin-7 antibody (clone KRT7/3733).~
IHC staining of FFPE human bladder carcinoma with Cytokeratin-7 antibody (clone KRT7/3733). HIER: boil tissue sections in pH 9 10mM Tris with

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CodeSizePrice
NSJ-V4278-20UG20 ug£264.00
Quantity:
NSJ-V4278-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Description:
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. mAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the Cytokeratin 7 antibody.
Limitation:
This Cytokeratin 7 antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P08729

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