NSJ Bioreagents

Phospho-ERK1/2 Antibody (pThr202/pTyr204)

Product Code:
 
NSJ-R20465
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM451
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant phospho-ERK1/2 antibody at -20°C.
1 / 2
Western blot testing of lysate from human A431 cells, non-treated (-) or treated (+) with EGF, using recombinant phospho-ERK1/2 antibody at a 1:1000 dilution. Expected molecular weight: ERK1 ~42 kDa, ERK2 ~43 kDa.~
2 / 2
IHC staining of FFPE h
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Western blot testing of lysate from human A431 cells, non-treated (-) or treated (+) with EGF, using recombinant phospho-ERK1/2 antibody at a 1:1000 dilution. Expected molecular weight: ERK1 ~42 kDa, ERK2 ~43 kDa.~
IHC staining of FFPE h

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NSJ-R20465-0.1ML100 ul£524.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1:100-1:200,Western blot: 1:500 -1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to human p44/42 MAPK (ERK1/2) only when phosphorylated at Thr202 and Tyr204. There is no cross-reactivity to Erk1/2 that is not phosphorylated.
Format:
Purified
Formulation:
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A phospho-peptide corresponding to human Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) was used as the immunogen for the phospho-ERK1/2 antibody.
Limitation:
This recombinant phospho-ERK1/2 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P27361, P28482

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