NSJ Bioreagents

Human Chimeric SARS-CoV-2 Nucleocapsid Protein Antibody (2019-nCov)

Product Code:
 
NSJ-R20480
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Chimeric (this antibody is recognized only by an anti-Human IgG secondary antibody)
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RMH03
Regulatory Status:
 
RUO
Target Species:
 
SARS-CoV-2
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Store the recombinant rabbit-human chimeric SARS-CoV-2 Nucleocapsid Protein antibody at -20°C.
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Binding curve of anti-NP (2019-nCov) monoclonal antibody RMH03 and recombinant 2019-nCov Nucleocapsid Protein. ELISA plate was coated with 50ng recombinant 2019-nCov Nucleocapsid Protein at concentration of 1ug/ml. A 2-fold serial dilution from 125 ng/mL was performed using recombinant rabbit-human chimeric
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Binding curve of anti-NP (2019-nCov) monoclonal antibody RMH03 and recombinant 2019-nCov Nucleocapsid Protein. ELISA plate was coated with 50ng recombinant 2019-nCov Nucleocapsid Protein at concentration of 1ug/ml. A 2-fold serial dilution from 125 ng/mL was performed using recombinant rabbit-human chimeric

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NSJ-R20480-100UG100 ug£524.00
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Further Information

Application Details:
Use at an assay dependent concentration
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant rabbit-human chimeric SARS-CoV-2 Nucleocapsid Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody is a Rabbit-Human Chimeric antibody made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of a rabbit anti-NP(2019-nCov) monoclonal antibody with the constant domain of Human IgG1. It reacts to Nucleocapsid Protein of SARS-CoV-2 (2019-nCov). No cross-reactivity to common SARS coronaviruses observed. It can be used as the detect antibody in a Sandwich ELISA assay with clone RM420 monoclonal antibody. This antibody is recognized only by an anti-Human IgG secondary antibody.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A recombinant 2019-nCov Nucleocapsid Protein expressed in E.coli was used as the immunogen for the recombinant rabbit-human chimeric SARS-CoV-2 Nucleocapsid Protein antibody.
Limitation:
This recombinant rabbit-human chimeric SARS-CoV-2 Nucleocapsid Protein antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant

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