Collagen Type I polyclonal antibody

Human

Liquid

Native purified human and bovine placenta Collagen Type I.

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Rabbit polyclonal antibody raised against native Collagen Type I.

ELISA (1:5000-1:50000)
FLISA (1:100)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:100)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.

Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

In 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% sodium azide).