Abnova Corporation

COL1A1 polyclonal antibody

Product Code:
 
PAB17204
Product Group:
 
Primary Antibodies
Supplier:
 
Abnova Corporation
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
 

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CodeSizePrice
PAB17204-100uL100 uL£488.00
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Further Information

Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
OI4
Gene Description:
collagen, type I, alpha 1
Gene ID:
1277
Gene Name:
COL1A1
Immunogen:
A synthetic peptide corresponding to human COL1A1.
Product Description:
Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.
Protein Accession:
P02452
Recommend Dilutions:
Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.
Specificity:
This antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes. specific to the propeptide portion of the ~180 KDa collagen I alpha1 polypeptide in human lung fibroblast extract. This antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Storage Buffer:
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)

References

  • http://www.ncbi.nlm.nih.gov/pubmed/15728585
  • http://www.ncbi.nlm.nih.gov/pubmed/2683783