anti-Rab6-GTP mAb (rec.) (AA2)
| Code | Size | Price |
|---|
| AG-27B-0004-C100 | 100 ug | £280.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Antibody Isotype: IgG2λ
Antibody Clonality: Recombinant Antibody
Antibody Clone: AA2
Regulatory Status: RUO
Target Species:
- Drosophila (fruit fly)
- Human
- Mouse
Application: Immunocytochemistry (ICC)
Shipping:
-20°C
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Ras-related Protein Rab-6
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu.
Long Description:
Recombinant Antibody. Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6•GDP. Species cross-reactivity: Drosophila, Human, Mouse. Clone: AA2. Isotype: Human IgG2lambda. Applications: ICC. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
NCBI, Uniprot Number:
P20340 (Rab6A
Other data:
anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Package Type:
Plastic Vial
Product Description:
Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
Purity:
>95% (SDS-PAGE)
Source / Host:
Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Specificity:
Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6•GDP.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
References
Recombinant antibodies to the small GTPase Rab6 as conformation sensors: C. Nizak, et al.; Science 300, 984 (2003) | Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking: E. Del Nery, et al.; Traffic 7, 394 (2006) | Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al; BMC Biotechnol. 10, 59 (2010)