AdipoGen Life Sciences

SuperKillerTRAIL™, Soluble (human) (rec.)

Product Code:
 
AG-40T-0002
Product Group:
 
Recombinant Proteins
Host Type:
 
E.coli
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Dry Ice
Storage:
 
-80°C
 

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AG-40T-0002-C02020 ug£347.00
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AG-40T-0002-30203 x 20 ug£717.00
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Further Information

Alternate Names/Synonyms:
TRAIL; Apo-2L; TNFSF10; CD253
Biological Activity:
Induces apoptosis at concentrations of >1ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. Shows superior activity on human cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).
Concentration:
0.5mg/ml
EClass:
32160000
Endotoxin:
<0.01EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT.
Handling Advice:
Avoid freeze/thaw cycles.
Long Description:
Protein. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge. Source: E. coli. Endotoxin content: <0.01EU/µg purified protein (LAL test; Lonza). Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT. Binds to human TRAIL receptors. Purity: >98% (SDS-PAGE). TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
NCBI, Uniprot Number:
P50591
Other data:
In order to retain full activity do not dilute higher than to a concentration of 0.1mg/ml, prepare 20µl aliquots and store at -80°C. Working dilution: 2-100ng/ml.
Package Type:
Plastic Vial
Product Description:
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
Purity:
>98% (SDS-PAGE)
Sequence:
The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
Source / Host:
E. coli
Specificity:
Binds to human TRAIL receptors.
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. , Stable for at least 1 year after receipt when stored at -80°C.

References

Functional TRAIL receptors in monocytes and tumor-associated macrophages: A possible targeting pathway in the tumor microenvironment: M. Liguori, et al.; Oncotarget 7, 41662 (2016) | Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory ?FADDosome? Complex upon TRAIL Stimulation: C.M. Henry & S.J. Martin; Mol. Cell 65, 715 (2017) | beta-Thujaplicin Enhances TRAIL-Induced Apoptosis via the Dual Effects of XIAP Inhibition and Degradation in NCI-H460 Human Lung Cancer Cells: S. Seno, et al.; Medicines 8, (2021) | Caspase-8 mutations associated with head and neck cancer differentially retain functional properties related to TRAIL-induced apoptosis and cytokine induction: Z. Cui, et al.; Cell Death Dis. 12, 775 (2021)