Further Information
K10; CK10; KPP; Cytokeratin 10; Epidermolytic Hyperkeratosis; Keratosis Palmaris Et Plantaris; Keratin, type I cytoskeletal 10
3h
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
31.25-2000pg/mL
48T, 96T, 96T?5, 96T?10, 96T?100
Keratin 10
Double-antibody Sandwich
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 10 (CK10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 10 (CK10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Tumor immunity;Infection immunity;Dermatology;
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
The minimum detectable dose of this kit is typically less than 12.2pg/mL
This assay has high sensitivity and excellent specificity for detection of Cytokeratin 10 (CK10).
No significant cross-reactivity or interference between Cytokeratin 10 (CK10) and analogues was observed.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 10 (CK10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 10 (CK10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 10 (CK10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokeratin 10 (CK10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
P13645